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KMID : 0903519990420030146
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1999 Volume.42 No. 3 p.146 ~ p.150
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Antagonism and Structural Identification of Antifungal Compound from Chaetomium cochliodes against Phytopathogenic Fungi
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Kang, Kyu Young
Kang, Jae Gon/Kim, Keun Ki
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Abstract
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As a part of the integrated disease system in greenhouse, an antifungal fungus(AF1) was isolated from grrenhouse soil. It exhibited strong inhibitory activites against Pythium ultimum, Phytophtora capsici, Phizoctonia solani, Botrytis cinerea, and Fusaium oxysporum based on dual culture on 1/5 strength of potato dextrose agar between antagonistic fungus and several plant pathogens. The antagomistic fungus was identified as Chaetomium cochliodes, based on morphological characteristics; the body of the perithecium bears straight or slightly wavy, unbranched hairs, whilst the apex bears a group of spirally coiled hairs. To investigate antagomistic principles, antifungal compound was extracted and fractionated by different solvant system. An antifungal compound was isolated as pure crystal from is culture filtrate using organic solvent extraction and column chromatography, followed bt preparative thin layer chromatography. The chemical structure of the purified antifungal compound was identified as chaetoglobosin A based on the data obtained form ¹H-NMR, ^(13)C-NMR, DEPT 90, 135, ¹H-¹H COSY, ¹H-^(13)C COSY and EI/MS. ED_(50) values of the chaetoglobosin A against P. ultimum, P. capsici, R. solani, B. cinerea and F. oxysporum were 1.98, 4.01, 4.16, 2.67 and 35.14 ppm, respectively.
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